Insights into the function of long noncoding RNAs in sepsis revealed by gene co-expression network analysis

Transcriptional profiling of neutrophyl granulocytes from 24 subjects was used to investigate their differences regarding sepsis and aging using gene network approaches. Two-color hybridization design. Aliquots of total RNA isolated from adult or elder individuals with sepsis or healthy controls were labeled with Cy3 and co-hybridized with a common reference RNA pool (Universal Human Reference RNA, Agilent, cat #740000) labeled with Cy5 to Agilent DNA SurePrint G3 Human Gene Expression 8x60k v2 Oligoarray (design ID # 039494) . Biological replicates: 6. No technical replicates.