Spatially Resolved RNA Sequencing in Micropatterned Tumor Models

MCF-7 breast cancer cells were micropatterned in circular regions surrounded by Bone Marrow Stromal Cells (BMSCs) in a micropatterned tumor-stromal assay (uTSA). After four days of culture, cancer cells from the edge and center of the micropattern were extracted with Laser Capture Microdissection (LCM), and their RNA was isolated and sequenced.Relevance: The goal of this project is to understand the heterogeneity of cell signaling in association with tumor architecture and tumor-stromal interactions in the tumor microenvironment (TME).