Hit reproducibility between experiments.
收藏Figshare2015-12-02 更新2026-04-29 收录
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1Microarray experiment where two technical replicates were combined for each biological replicate in Rosetta Resolver to identify hits (Fold change >2, p≤0.05).2Microarray experiment where two technical and two biological replicates (A & B) were combined for either S100 screen with 100 copies per shRNA in PCR or S500 screen with 500 copies per shRNA in PCR using Rosetta Resolver to identify hits (Fold change >2, p≤0.05).3Next generation experiment where one biological replicate (no technical replicates) was analyzed using DESeq to identify hits (Fold change >2, p≤0.05).4Next generation experiment where two biological replicates (A & B) were combined for either S100 screen with 100 copies per shRNA or S500 screen with 500 copies per shRNA using DESeq to identify hits analyzed using DESeq to identify hits (Fold change >2, p≤0.05).5Next generation experiment where two biological replicates (A & B) were combined for either S100 screen with 100 copies per shRNA or S500 screen with 500 copies per shRNA using DESeq to identify hits analyzed using DESeq to identify hits (Fold change >2, p≤0.05). For comparison, hits were filtered to only include hits that were detectable on the microarray.
创建时间:
2015-12-02



