Histone H3 modifications ChIP on chip

Chromatin modification through the covalent modifications of histones play crucial role on establishment and propagation of gene expression pattern. Here we sought a global view of histone H3 modifications (tri-M-K4/27 and di-M-K27/36) and occupancy in Arabidopsis thaliana using ChIP combined with high-density tiling microarrays. In these analyses, we included vip3 mutant plants as well to gain an insight into role of Paf1C in plants. Keywords: ChIP on chip Genomic DNAs associated with histone H3 K4 trimethylation (TMK4), H3 K36 dimethylation (diMK36), H3 K27 dimethylation (diMK27), H3 K27 trimethylation (TMK27), and H3 from wild type (WT) and vip3 mutant plants (vip3) were analyzed using ChIP on chip technology