Development of outbred CD1 mouse colonies with distinct standardized gut microbiota profiles.. mouse gut metagenome

Outbred CD1 mouse colonies were generated to be colonized with four distinct complexgut microbiota (GM) profiles representing contemporary rodent producer profiles. Mice were maintained atthe University of Missouri for nine generations and fecal samples were analyzed using 16S rRNA gene sequencing.Additionally, ninth generation mice were shipped to collaborating institutions and fecal samples were collectedand sequenced for detection of post-shipping associated shifts in GM profile.