Mutants tested for initial sensitivity and development of acute functional tolerance to ethanol.

aThe degree of initial sensitivity was determined as the percent of untreated speed after 10 minutes of ethanol exposure. Significance was determined relative to the effect of ethanol on wild-type N2 animals treated on the same plates using paired two-tailed t-tests. bThe development of acute functional tolerance (AFT) was determined by comparing the rate of locomotion at 30 minutes vs. 10 minutes of ethanol exposure. If the mutant strain developed significant tolerance, we compared its rate of development of tolerance to the rate of wild-type N2 animals treated on the same plates using unpaired two-tailed t-tests. cida-1 is known to be negatively regulated by pag-3; the response of the strong loss of function ida-1(ok409) mutant animals to ethanol was indistinguishable from that of N2, suggesting that a change in neurosecretion is not the explanation for the AFT phenotype of ctbp-1 and pag-3. dGenetic manipulation of fat stores by mutations in the genes nhr-49, bbs-1, tub-1, fat-7 and fat-5, and sbp-1 did not alter ethanol sensitivity or AFT in a consistent manner, suggesting that fat levels themselves do not influence the rate of development of AFT. esbp-1 mutant animals have a basal speed that is significantly slower than N2 (Table S1). For this reason, the magnitude and interpretation of the ethanol responses of this strain may be subject to a floor effect.