Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth [bulkRNAseq_AKPS]
收藏NIAID Data Ecosystem2026-05-01 收录
下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE207069
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资源简介：
Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Bulk RNA-sequencing was performed on STAR populations after 5 days and after 12 days of plating single STAR+ or STAR- cells. Organoids were grown from single STAR+ or STAR- cells (APCKO/KO; KRASG12D/-; TP53KO/KO; SMAD4 KO/KO (AKPS)). After 5 days and after 12 days, organoids were harvested, dissociated to single cells, and between 10-20 k STAR+ and STAR- cells were collected in technical replicates for bulk RNA-seq.
创建时间：
2023-12-01
