Hitdb V1.00 For Dada2

HITdb is a reference taxonomy for Human <strong>Intestinal</strong> 16S rRNA genes as described in Ritari J, Salojärvi J, Lahti L &amp; de Vos WM. Improved taxonomic assignment of human intestinal 16S rRNA sequences by a dedicated reference database. BMC Genomics. 2015 Dec 12;16(1):1056. doi: 10.1186/s12864-015-2265-y. This is version converted to be used with dada2 (http://benjjneb.github.io/dada2). After extracting your sequence table you can use HITdb via: taxa_hdb &lt;- assignTaxonomy(seqtab_nochim, "hitdb_v1.00.fa.gz")<br> colnames(taxa_hdb) &lt;- c("Phylum", "Class", "Order", "Family", "Genus", "Species") Please be aware that HITdb is specific for intestinal samples, thus it might give you arbitrarily wrong results for non-intestinal samples.<br>