Saccharum species Raw sequence reads

Spore suspension of Ssc was inoculated at the growth point of ZZ1, at the young roots and buds, respectively. Inoculated plants were placed in a constant temperature incubator in a medium moisturizing culture. Smut group samples were collected at 5d and 20d after inoculation, with water as a control treatment. The roots and buds of ZZ9 were collected at 0d, 1d, 2d, 3d, and 4d for each of the above treatments with three duplicates. Total RNA was extracted from the above samples using RNAprep Pure plant Kit and subsequently used for cDNA library construction. The quality of the cDNA library was assessed on the Agilent Bioanalyzer 2100 system and sequenced on an Illumina Novaseq platform.