Expression data from prostate cancer LNCaP-AdtHs cells in response to androgen, ADT, normoxia, or hypoxia

Androgen and hypoxia are two cancer hallmark pathways; both exert biological effects primarily via the regulation of gene expression We used gene expression array to determine the global gene expression changes due to androgen, castration (androgen-free) in normoxia and hypoxia conditions. Microarray assays were performed in the OHSU Gene Profiling Shared Resource Androgen responsive human prostate cancer LNCaP-AdtHs cells were cultured in four conditions: with 5% charcoal-stripped FBS (CSS) (ADT), 5% CSS + 1nM synthetic androgen R1881 (R1881) in 20% oxygen (normoxia) or in 1% oxygen (hypoxia).