S3_qPCR_let-7_alleles_validation

Raw qPCR data supporting the Fig. S3. This entry contains three datasets: FigS3B_qPCR_xe150 validation_mature: The levels of mature let-7 and sn2841 (reference gene) were measured by RT-qPCR using the TaqMan chemistry in the strains listed below at 30 and 33 hours of growth at 25°C (starting from synchronized L1 population). Only unbalanced let-7(-) animals were used for the experiment. Condition Strain code Genotype wild type N2 wild type let-7(-) HW2569 let-7(xe150) X; xeEx365[let-7p::let-7::SL1_operon::gfp, unc-119 (+); rab-3p::mCherry; myo-2p::mCherry; myo-3p::mCherry] FigS3C_qPCR_xe150 validation_primary: The levels of primary let-7 transcript and actin mRNA (reference gene) were measured by RT-qPCR in the strains listed below at 30 and 33 hours of growth at 25°C (starting from synchronized L1 population). Only unbalanced let-7(-) animals were used for the experiment. Condition Strain code Genotype wild type N2 wild type let-7(-) HW2569 let-7(xe150) X; xeEx365[let-7p::let-7::SL1_operon::gfp, unc-119 (+); rab-3p::mCherry; myo-2p::mCherry; myo-3p::mCherry] FigS3D let-7 BS: The levels of mature let-7 and sn2841 (reference gene) were measured by RT-qPCR using the TaqMan chemistry in the strains listed below at 12 and 15 hours of growth at 25°C (starting from synchronized L1 population). Condition Strain code Genotype wild type N2 wild type let-7(∆BS) HW3343 let-7(xe336[GGAGmut]) X lin-28(0) HW2705 lin-28(xe192) I