A list and description of Rab isolates created for the C. elegans ORFeome-based toolkit.

WT, DN and CA clones included in the Rab Toolkit are given isolate names otherwise an explanation for its absence is provided. Subfamily classifications are based on Diekmann et al. 2011 [33] and/or data presented here. The majority of C. elegans rab genes are predicted to have only one splice variant with the following exceptions: WormBase describes two splice variants for rab-3 that code for proteins 233 and 219 amino acids (aa) in length. ORFeome project primers were designed to amplify the shorter isoform only. WormBase describes two splice variants for 4R79.2 (Rab44) that code for proteins 311 aa and 395 aa in length. ORFeome project primers were designed to amplify the longer isoform only. Names listed under “other” are from WormBase or Pereira-Leal and Seabra (2001). Finally, while rab-37 shows 100% identity with the Refseq protein NP_001041293 it contains an additional 5 amino acids at its N-terminus. See text for details.