p53 Chip-Seq in U2OS treated with UV. p53 Chip-Seq in U2OS treated with UV

We report that TAF1 phosphorylates p53 at Thr55 on the p21 promoter and this phosphorylation leads to dissociation of p53 from the promoter. Indeed, ChIP-Seq analysis reveals p53 undergoes promoter dissociation at a global level after response to DNA damage, underscoring general nature of the regulation. UVC treatment at the time points indicated was used to access p53 transcription factor signal induction and termination.