The involvement of RIPK4 in TNF-α-stimulated IL-6 and IL-8 production by melanoma cells

The receptor-interacting protein kinase 4 (RIPK4) plays an oncogenic role in melanoma by regulating NFκB and Wnt/β-catenin pathways. As its role in melanoma remains not fully understood, we examined the effects of its downregulation on melanoma transcriptomics. Using RNA-seq, we observed broad transcriptome changes in WM266.4 cells upon RIPK4 downregulation, indicating a complex role for RIPK4 in regulating adhesion, migration, proliferation, and inflammatory processes in melanoma cells. Furthermore, our study highlights the potential functional partners of RIPK4, such as BIRC3, TNF-α receptors, and MAP2K6. To investigate the effects of RIPK4 silencing on melanoma cell fate, we silenced RIPK4 in WM266.4 human melanoma cells. Subsequently, we performed bulk RNA-seq to examine the transcriptional changes using gene expression profiling analysis. The experiment comprised three replicates.