Summary of ³¹P NMR chemical shifts observed in culture supernatants of various <i>phn</i> strains grown with various organophosphonates.^a

aData are from samples taken after 20 h of incubation in the presence of phosphonate. Due to uneven acidification of the growth media chemical shifts occasionally showed small differences among otherwise similar cultures. Particularly 2NAcAmEtPn was prone to pH dependent chemical shifts (up to 0.6 ppm). bThis signal was assigned as NAcAmMePn by spiking the NMR sample with the reaction product of aminoalkylphosphonate N-acetyltransferase with AmMePn as the acetyl acceptor. cThis signal was assigned as 2NAcAmEtPn by spiking the NMR sample with the reaction product of aminoalkylphosphonate N-acetyltransferase with 2AmEtPn as the acetyl acceptor. dThis signal was assigned as S1NAcAmEtPn by spiking the NMR sample with the reaction product of aminoalkylphosphonate N-acetyltransferase with S1AmEtPn as the acetyl acceptor. eS1NAcAmEtPn, N-acetyl-(S)-1-aminoethylphosphonate. fRib1′S1NAcAmEtPnf, α-d-ribosyl 1′-(N-acetyl-(S)-1-aminoethylphosphonate).