Dataset of SARS-CoV-2 wastewater data from Älvkarleby, Sweden

This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Älvkarleby, Sweden. The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of Agricultural Sciences) and her research groups in collaboration with Uppsala Vatten. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.The viral content is concentrated according to the protocol from Ahmed et al. https://doi.org/10.1016/j.scitotenv.2020.139960according to the modified electronegative filtration method. Viral RNA is extracted and SARS-CoV-2 gene copy nr quantified using R-qPCR. Recovery efficiency is measured using Bovine coronavirus (BCoV) as a virus surrogate. Pepper mild mottle virus (PPMoV) is used to correct for variations in water size and flow. For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsalaThe dataset for Älvkarleby started week 14 2021.The dataset is available as part of the Environmental Virus Profiling data section. The amount of SARS-CoV-2 virus in wastewater across Sweden" https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsala on the Swedish COVID-19 Data Portal (https://covid19dataportal.se). The dataset is also available as part of the CRUSH Covid project https://crush-covid.shinyapps.io/crush_covid/Funded by SciLifeLab/KAW National COVID-19 Research Program https://www.scilifelab.se/covid-19/national-program/

此为瑞典亚尔瓦卡尔莱比市持续更新之SARS-CoV-2病毒RNA数据集的元数据记录。该数据集系由瑞典农业大学（SLU）副教授安娜·J·塞凯利（Anna J. Székely）及其研究团队联合乌普萨拉水务公司（Uppsala Vatten）共同开展的研究项目之成果。该研究团队隶属于SciLifeLab国家COVID-19研究计划的环境病毒分析研究区域。病毒含量根据Ahmed等人的方案（https://doi.org/10.1016/j.scitotenv.2020.139960）进行浓缩，采用改进的电负性过滤法。病毒RNA通过R-qPCR技术提取，并量化SARS-CoV-2基因拷贝数。以牛冠状病毒（BCoV）作为病毒替代品，测量回收效率。以辣椒花叶病毒（PPMoV）校正水体体积和流量带来的变异。详细信息请参阅https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsala。该数据集自2021年第14周起开始收集，并作为环境病毒分析数据部分提供。瑞典全国范围内废水中的SARS-CoV-2病毒含量数据亦可通过瑞典COVID-19数据门户（https://covid19dataportal.se）获取。此外，该数据集亦为CRUSH Covid项目（https://crush-covid.shinyapps.io/crush_covid/）的一部分，该项目由SciLifeLab/KAW国家COVID-19研究计划资助（https://www.scilifelab.se/covid-19/national-program/）。