Effect of feeding on gene expression in HEK-293E cells either treated with Torin1 or not.

We examined how mTORC1 influences mRNA expression and alternative splicing during a growth response in human embryonic kidney (HEK) 293E cells, in which mTORC1 is not hyperactive. We released a starvation-induced growth arrest by refeeding, then analyzed mRNA levels and alternative splicing events one and four hours later under either control or Torin1 treatment conditions. Overall design: To investigate the nutrient response on gene expression, we treated HEK-293E cells with either 1 or 4 hours of feeding, with or without Torin1 treatment.