Supplementary Figure 5, (d) of the paper Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions.
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https://researchdata.edu.au/supplementary-figure-5-cadherin-junctions/3368742
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Images show western blots results of co-immunopecipitation experiments between ROCK-1 and NMIIA or ROCK-1 and the ROD domain of NMIIA. HEK293T cells were transfected with GFP-FL NMIIA (full-length) or GFP-NMIIA-ROD and treated with Y-27632 (30 μM, 1 hour). Immunoprecipitation was performed using GFP-TRAP and lysates were immunoblotted for ROCK-1 and GFP.
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The University of Queensland
