Bacteria Raw sequence reads

Microbial collections based exclusively on axenic cultures are time consuming, costly and may result in the loss of relevant biological information, since there are microorganisms that might depend on microbe-microbe interactions for their growth. As an alternative, we explore the concept of community-based culture collections that is based on the isolation of colonies containing single or multiple microorganisms. By using this approach, we created a community-based culture collection with isolates from sugarcane plant organs. We developed a method to annotate the community-based isolates through a multiplex strategy for pooling and sequencing near-full-length ribosomal gene amplicons. These method provide a high-throughput strategy for identifying isolated microbes in culture collection and allow fast and accurate cross-referencing with culture-independent community profiling. This dataset contain reads from five plates that were used to the development of these methodologies.