Raw data of qRT-PCR and western blot analyses of proteasome subunits and GFP-CL1 degradation in Podospora anserina

Dataset 1 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 1ACP values of the reference gene PaPorin and of the target gene PaPre3 are displayed for juvenile middle-aged and senescent samples
Dataset 2 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 1ACP values of the reference gene PaPorin and of the target gene PaPre2 are displayed for juvenile middle-aged and senescent samples
Dataset 3 : Raw data of qRT-PCR analysis of the PaUmp1 gene used in Figure 1ACP values of the reference gene PaPorin and of the target gene PaUmp1 are displayed for juvenile, middle-aged and senescent samples
Dataset 4 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 2ACP values of the reference gene PaPorin and of the target gene PaPre3 are displayed. The wild type CP is the mean CP value of juvenile samples displayed in Dataset 1
Dataset 5 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 2BCP values of the reference gene PaPorin and of the target gene PaPre2 are displayed. The wild type CP is the mean CP value of the juvenile samples displayed in Dataset 2
Dataset 6: Raw data of western blot displayed in Figure 1B probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM (Cat# 26616) Prestained protein ladder. Lanes 2 – 7: Samples described in Figure 1B.
Dataset 7: Raw data of western blot displayed in Figure 1B probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 7 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B.
Dataset 8: Raw data of western blot displayed in Figure 1B probed with α-PaPUP1. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPUP1. Red signal represents fluorescence at 700 nm. Lane 10 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B. Lanes 7 - 9 are not relevant to this study.
Dataset 9: Raw data of western blot displayed in Figure 1B probed with α-PaHSP60. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-PaHSP60. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 2 - 7: Samples described in Figure 1B.
Dataset 10: Raw data of western blot displayed in Figure 2C probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 – 5 and lane 9: Samples described in Figure 1C. The other lanes are not relevant to this study.
Dataset 11: Raw data of western blot displayed in Figure 2D probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study.
Dataset 12: Raw data of western blot displayed in Figure 2D probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study.
Dataset 13: Raw data of western blot displayed in Figure 3B probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 9 and 10: Samples described in Figure 3B.
Dataset 14: Raw data of western blot displayed in Figure 3C probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 5 and 6: Samples described in Figure 3C.

数据集 1：PaPre3 基因 qRT-PCR 分析的原始数据，如图 1 所示，展示了参照基因 PaPorin 及目标基因 PaPre3 在幼年、中年和衰老样本中的 ACP 值。

数据集 2：PaPre2 基因 qRT-PCR 分析的原始数据，如图 1 所示，展示了参照基因 PaPorin 及目标基因 PaPre2 在幼年、中年和衰老样本中的 ACP 值。

数据集 3：PaUmp1 基因 qRT-PCR 分析的原始数据，如图 1 所示，展示了参照基因 PaPorin 及目标基因 PaUmp1 在幼年、中年和衰老样本中的 ACP 值。

数据集 4：PaPre3 基因 qRT-PCR 分析的原始数据，如图 2 所示，展示了参照基因 PaPorin 及目标基因 PaPre3 的 ACP 值。野生型 CP 值为数据集 1 中幼年样本的平均 CP 值。

数据集 5：PaPre2 基因 qRT-PCR 分析的原始数据，如图 2 所示，展示了参照基因 PaPorin 及目标基因 PaPre2 的 BCP 值。野生型 CP 值为数据集 2 中幼年样本的平均 CP 值。

数据集 6：如图 1B 所示的蛋白质印迹原始数据，使用 α-PaPRE2 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPRE2 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯（Cat# 26616）。第 2 条带至第 7 条带：如图 1B 所述的样本。

数据集 7：如图 1B 所示的蛋白质印迹原始数据，使用 α-PaPRE3 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPRE3 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 7 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 1 条带至第 6 条带：如图 1B 所述的样本。

数据集 8：如图 1B 所示的蛋白质印迹原始数据，使用 α-PaPUP1 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPUP1 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 10 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 1 条带至第 6 条带：如图 1B 所述的样本。第 7 条带至第 9 条带与本研究无关。

数据集 9：如图 1B 所示的蛋白质印迹原始数据，使用 α-PaHSP60 进行探针。在 700 nm 处检测荧光。红色信号代表在 700 nm 处由与 α-PaHSP60 结合的抗鼠 700 抗体引起的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 2 条带至第 7 条带：如图 1B 所述的样本。

数据集 10：如图 2C 所示的蛋白质印迹原始数据，使用 α-PaPRE3 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPRE3 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 3 条带至第 5 条带和第 9 条带：如图 1C 所述的样本。其他条带与本研究无关。

数据集 11：如图 2D 所示的蛋白质印迹原始数据，使用 α-PaPRE2 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPRE2 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 3 条带和第 4 条带：如图 1D 所述的样本。第 2 条带与本研究无关。

数据集 12：如图 2D 所示的蛋白质印迹原始数据，使用 α-PaPRE3 进行探针。在 700 nm 和 800 nm 处检测荧光。两个信号均在显示图像中合并。绿色信号代表在 800 nm 处产生的荧光，由与 α-PaPRE3 结合的抗兔 800 抗体引起。红色信号代表 700 nm 处的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 3 条带和第 4 条带：如图 1D 所述的样本。第 2 条带与本研究无关。

数据集 13：如图 3B 所示的蛋白质印迹原始数据，使用 α-GFP 进行探针。在 700 nm 处检测荧光。红色信号代表在 700 nm 处由与 α-GFP 结合的抗鼠 700 抗体引起的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 9 条带和第 10 条带：如图 3B 所述的样本。

数据集 14：如图 3C 所示的蛋白质印迹原始数据，使用 α-GFP 进行探针。在 700 nm 处检测荧光。红色信号代表在 700 nm 处由与 α-GFP 结合的抗鼠 700 抗体引起的荧光。从左至右的第 1 条带：Thermo Fischer PageRulerTM 预染蛋白梯。第 5 条带和第 6 条带：如图 3C 所述的样本。