mtDNA genome assembly result using different assembler.

[1] Rows noted by “#” are speciemen for which the adaptor sequence could not be detected on read2 by Fastp (v0.24.0). [2] Number of raw reads before filtering. [3] Reference sequeces used in MitoBim and the mapping-consensus approach. ***/**/* notes that the sequences are of the same species/genus/family as the specimen. [4] The top blast results of these 8 scaffolds are: Timema bartmani (83.3%), Campylobacter jejuni (99.9%), Ignavibacterium album JCM 16511 (85.71%), Papio anubis (73.91%), Sus scrofa (77.45%), Naegleria fowleri (86.54%), Danio rerio (73.33%), and Caudoviricetes sp. (92.5%). [5] Two specimens’ assembled scaffolds ($) were subjected to BLAST search (blastn) on NCBI, but no significant matches were returned. (XLSX)