Data from: Loss of miR-143 and miR-145 in condyloma acuminatum promotes cellular proliferation and inhibits apoptosis by targeting NRAS

The expression profile of miRNAs and their function in condyloma
acuminatum (CA) remains unknown. In this study, we aimed to detect the
effects of miR-143 and miR-145, the most downregulated in CA samples using
high-throughput sequencing, on cell proliferation and apoptosis, to
determine a novel therapeutic target for CA recurrence. RT-qPCR was used
to validate the lower expression of miR-143 and miR-145 in a larger size
of CA samples, and the expression of NRAS in CA samples was significantly
higher than self-controls as determined western blotting assay. Luciferase
assay was performed to confirm that miR-143 or miR-145 targeted NRAS
directly. Transduction of LV-pre-miR-143 or LV-pre-miR-145 to human
papilloma virus (HPV)-infected SiHa cells led to reduced proliferation,
greater apoptosis, and inhibition of expression of NRAS, PI3Kp110α, and
pAKT. However, knockout of miR-143 or miR-145 in human epidermal
keratinocytes (HEKs) by delivery of CRISPR/CAS9-gRNA for target miRNAs
protected cells from apoptosis and upregulated expression of target genes
as described above. MiR-143 and miR-145 sensitized cells to nutlin-3a, a
p53 activator and MDM2 antagonist, while their loss protected cells from
the stress of nutlin-3a. Furthermore, siRNA targeting NRAS showed similar
effects on proliferation and apoptosis as miR-143 or miR-145. Taken
together, our results suggest that loss of miR-143 or miR-145 in CA
protects HPV-infected cells from apoptosis induced by environmental
stress, in addition to promoting cellular proliferation and inhibiting
apoptosis by targeting NRAS/PI3K/ATK. Restoration of miR-143 or miR-145
might provide an applicable and novel approach to block the recurrence and
progression of CA.