Datasets for 'Label-free imaging of 3D pluripotent stem cell differentiation dynamics on chip'

Here we publish the datasets associated with our publication ‘Label-free imaging of 3D pluripotent stem cell differentiation dynamics<br> on chip’. 3D cultures of human induced pluripotent stem cells (hiPSCs) were imaged while undergoing definitive endoderm (DE) differentiation.<br> Data were acquired either on the live 3D cultures at various time points during the 3 days DE differentiation, or after fixation and immunostaining, with the 3D cultures being fixed at regular 24 h intervals during differentiation, as to form a timeline. Images were recorded with a standard confocal microscope using a xy-resolution of 0.25 μm/px and a z-resolution of 1 μm/plane.