Neuropsin tetracycline-transactivator expression: horizontal sections (case 2877)

Bright-field microscopy images of serial horizontal brain sections showing neuropsin (Nop) promoter expression in a bigenic Nop-tetracycline-transactivator (tTA) mouse brain (case 2877, adult male), using a driver-reporter construct in which the Nop-tTA promoter regulates the expression of the E. coli derived LacZ reporter gene encoding β-galactosidase, which is visualized histologically using X-gal (5-Bromo-4-chloro-3-indolyl β-d-galactopyranoside) as a substrate. Methodological details are provided in Yetman et al., Brain Struct Funct 221:2231-49, 2016. Our data show that the Nop-tTA promoter mainly is expressed in the entorhinal cortex, but also in several other cortical regions.