Influence of CD5 Ala471Val polymorphism on TCR/CD3-mediated cytokine release.

PBMCs from healthy volunteers homozygous for the rs2241002-rs2229177 CD5 haplotypes Pro224-Ala471 (CC; n = 3) or Pro224-Val471 (CT; n = 4) were stimulated with different concentrations of OKT3 (0.1, 1.0 and 10 ng/mL) for 72 or 120 h. Cytokine levels in culture supernatants were measured in triplicate by a Luminex technique and the results expressed in pg/mL as mean ± S.D. Statistical analysis was performed using a one-tailed Mann-Whitney test, with confidence intervals of 95%. (*) full statistical significance (p0.05).Influence of CD5 Ala471Val polymorphism on TCR/CD3-mediated cytokine release.