Comparative RNA-sequencing of Brachypodium distachyon wild-type and bdmute developing leaf zones

Comparative RNA-sequencing of the developmental leaf zones in Brachypodium distachyon wild type and bdmute mutants that do not form stomatal subsidiary cells was performed. The aim was to identify genes relevant for subsidiary cell formation in B. distachyon. Overall design: 25 developmental zones (3mm at base of 2nd leaves) were analyzed per replicate and genotype. 3 replicates per genotype were sequenced, WT1, WT2, WT5 and sid1, sid2, sid5. WT corresponds to the reference genotype and sid (=bdmute) corresponds to the mutant genotype.