Mouse lens gene expression profile based on cDNA microarray analysis

To determine the relative gene expression profile in the lens tissue, total RNA extracted from the neonatal (P7) and adult (P60) mouse lenses were subjected to cDNA microarray analysis using the Affymetrix Mouse Genome 430 2.0 array. The expressed genes were annotated and organized them from their relative high to low expression. The genetic background of mouse strain used was C57/BL6. Total RNA was extracted from the pooled mouse lenses and their respective quality and quantity was evaluated with standards techniques. About 500 ng of total RNA at a concentration of approximately 50 ng/ml was used for the hybridization为解析小鼠晶状体组织的相对基因表达谱，研究人员从新生（出生后第7天，P7）与成年（出生后第60天，P60）小鼠的晶状体中提取总RNA，采用Affymetrix小鼠基因组430 2.0芯片（Affymetrix Mouse Genome 430 2.0 array）开展cDNA微阵列分析。对检测到的表达基因进行注释，并按相对表达量从高到低排序。所用小鼠品系的遗传背景为C57/BL6。总RNA提取自混合的小鼠晶状体样本，并通过标准实验技术对其质量与浓度进行评估。取浓度约50 ng/ml的总RNA约500 ng用于杂交反应。