Concentration of oligomer and monomer in Abeta preparations as determined by ELISA.

Quantification of relative concentrations of oligomeric and monomeric peptide in a freshly prepared solution of Abeta 1–42 (corresponding to lane 1 in Fig. 2A, 3 µM total Abeta concentration), Abeta 1–42 oligomers (corresponding to lane 2 in Fig. 2A, 3 µM total Abeta concentration) and Alzheimer's patient-derived Abeta (Fig. 3) measured by two-site (total) and single-site (oligomer) ELISA [4], [5], [39]. Both synthetic peptide solutions contain monomer. In contrast, Abeta peptide immunopurified from a 1 gram tissue sample of human Alzheimer's patient hippocampus contains no detectable monomer.
Concentration of oligomer and monomer in Abeta preparations as determined by ELISA.