The transcriptomic data of silver carp stomach and pyloric ceca

This study employs integrated transcriptomic and metabolomic approaches to elucidate the molecular and metabolic adaptation mechanisms in the stomach and pyloric ceca of mandarin fish (Siniperca chuatsi) to artificial feed, aiming to improve feed utilization efficiency. Methods: Juvenile mandarin fish were divided into live feed (LF) and artificial diet (AD) groups for six weeks, after which stomach and pyloric ceca tissues were collected for transcriptomic and metabolomic analyses. Results: Histological examination showed that the AD group exhibited significantly reduced mucosal and muscular layer thickness with submucosal thickening in the stomach, while the pyloric ceca developed irregular morphology compared to the regular circular shape in the LF group, without significant inflammation. Transcriptomic analysis revealed 2,513 upregulated (including pklr, akr1a1, and gstt2) and 2,552 downregulated (including pck1 and sec61a1) genes in the stomach of artificial diet. Pathway enrichment analysis indicated activation of glycolysis/gluconeogenesis and glutathione metabolism. In the pyloric ceca, 1,327 genes were upregulated and 2,054 were downregulated, with significant enrichment in oxidative phosphorylation, endoplasmic reticulum protein processing, and glycosaminoglycan degradation pathways. Protein-protein interaction network analysis identified hspa12a and phgdh as key regulatory genes.