Vibrio anguillarum MVAV6203 Genome sequencing. Vibrio anguillarum strain:MVAV6203

In this work, we sequenced the genome of V. anguillarum MVAV6203 by 454, Illumina Solexa method and splicing of PCR. The genome of V. anguillarum MVAV6203, with the GC content of 44.5 %, contains two chromosomes, the big one, termed as Chr1 is 3,097,087 bp and contains 2838 genes, the small one, termed as Chr2 is approximately 950 kb and encodes about 1000 genes. Genome sequence analysis indicated that Chr1 contains a number of the virulence related genes such as haemolysin, flagellin and out membrane protein. Most of the environmental adaptability genes like temperature stress, osmotic pressure and drug resistance are located on Chr2.V. anguillarum MVAV6203 chromosome 2 contains a super-integron with the length of about 60 kb and including 50~80 gene cassettes, most of the gene cassettes encode conservative hypothetical proteins. Several gene cassettes carry the genes related to Bleomycin resistance and RelBE Toxin-Antitoxin system which strengthen the stability of super-integron gene cassette array. Comparative genomics analysis demonstrated that V. anguillarum MVAV6203 has a close evolutionary relationship to Vibrio cholerae and Vibrio vulnificus, and simultaneously, its virulence regulation, pathogenic mechanism and drug resistance are similar to other vibrios strains. Comparative genomics analysis also determined 1895 important homologous proteins in vibrios.