Computational IHC-H&E mapping in mouse models of colitis (IHC WSIs from paired H&E-IHC WSIs, Part 1/2)

Inflammatory Bowel Disease (IBD) is characterized by chronic, dysregulated
inflammation in the gastrointestinal tract. The heterogeneity of IBD is
reflected through two major subtypes, Crohn’s Disease (CD) and Ulcerative
Colitis (UC). CD and UC differ across symptomatic presentation, histology,
immune responses, and treatment. While colitis mouse models have been
influential in deciphering IBD pathogenesis, no single model captures the
full heterogeneity of clinical disease. The translational capacity of
mouse models may be augmented by shifting to multi-mouse model studies
that aggregate analysis across various well-controlled phenotypes. Here,
we evaluate the value of histology in multi-mouse model characterizations
by building upon a previous pipeline that detects histological disease
classes in hematoxylin and eosin (H&E)-stained murine colons.
Specifically, we map immune marker positivity across serially-sectioned
slides to H&E histological classes across the dextran sodium
sulfate (DSS) chemical induction model and the intestinal
epithelium-specific, inducible Villin-CreERT2;Klf5fl/fl
(Klf5ΔIND) genetic model. In this study, we construct the beginning
frameworks to define H&E-patch-based immunophenotypes based on
IHC-H&E mappings.