RNA sequencing of Rbpm1 knockout in Plasmodium yoelii. Plasmodium yoelii

An RNA binding protein RBPm1 regulates the axoneme assembly in male gametocytes of Plasmodium yoelii. To understand the molecular mechanisms underlying this process, we performed RNA-Seq to examine the male transcriptome changes due to the loss of RBPm1. We used a DFsc7 reporter strain, which specifically expressed GFP in the male gametocytes, to obtain high-purity male gametocytes. We deleted the Rbpm1 in the DFsc7 strain and generated a mutant strain. Male gametocytes were sorted from DFsc7 and mutant strains by fluorescence-activated cell sorting (FACS) according to GFP fluorescence intensity and subjected to RNA-seq.