Evolution of gene expression during an adaptive diversification event

During a 1,000 generations evolution experiment, two types of bacteria (S and L) repeatedly diverged from a common ancestor (A) in a fully sympatric seasonal environment containing glucose and acetate. We compare the transcription profile of the two derived types and the common ancestor in order to investigate the metabolic modifications associated with this adaptive diversification event. Keywords: time point, experimental evolution Total RNA was extracted at 4 different time points of the 24H kinetic growth curve of each of the 3 types of bacteria (S, L and A). At each time point, S and L transcription profiles were compared to A. Each comparison was performed in 5 biological replicates.