Whole-transcript expression array analysis of intestinal epithelial cells from vehicle, TNBS-induced colitis BALB/cJ mice treated with vehicle or I3C

Whole-transcript expression array analysis of intestinal epithelial cells from TNBS-induced colitis mice treated with vehicle or I3C demonstrated several differentially expressed genes Downregulation of AhR expression in colitis mice was associated with downregulation of various antimicrobial peptides such as α-defensin 1, β-defensin1, reg1, reg4, reg3a and reg3b. However, treatment with I3C, an AhR ligand, increased the expression of AhR and AMPs such as α-defensin 1 For TNBS-induced colitis model, the animals were randomized into experimental and control groups and divided into 4 groups. These included: 1) naïve 2) TNBS, 3) TNBS+I3C, and 4) I3C alone. Intestinal epitheial cells (IECs) were isolated from ileal tissue of control and experimental animals and identified by EPCAM(+) staining using flow cytometry