A 3-D Printed Vitrification Device Integrated with French Straws

This data set includes ‘stl’ files that can be used for 3D printing, and ‘ipt’ files that can be used for design modifications in Autodesk Inventor or Fusion 360. More information of this device can be found in the article ‘A 3-D Printed Vitrification Device Integrated with French Straws’ published in the journal Hardware X.
(1) Vitrification wands:
Vitrification quality is inversely related to sample volume, because higher thermal mass leads to lower cooling rates. Thus, vitrification wands were designed with multiple configurations that differed by loop length and height (different number of layers in 3-D printing slicing). The nominal height per layer was 0.2 mm when printed with 0.4-mm printer nozzles. Loop configurations included three lengths (Short:15.4 mm, intermediate:16.6 mm, and long:24.4 mm) and three heights (1 layers, 2 layers, 3 layers). The total length of the wands was identical to French straws to fit within standard cryogenic goblet storage containers. Wands were printed with polylactic acid (PLA) thermoplastic filament, widely used for 3D printing, and can withstand liquid nitrogen temperatures.
(2) Jig apparatus for creation of protective sleeves:
A self-contained jig apparatus was designed to size, indent, and cut a standard 0.5-mL cryopreservation straw (IMV Technologies, L'Aigle, France) into two protective sleeves. This apparatus consisted of a dock, a stylus, and a stencil. The stylus featured a tapered tip to produce a defined indentation on French straws that would slide along a groove fabricated in the vitrification wands. The stencil ensured correct positioning of the French straws within the jig and facilitated localization of the indentation. The stylus enabled positioning of a razor blade, stylus, and stencil during sleeve making. The stylus and stencil were self-contained by insertion into the dock after use.

本数据集包含可用于3D打印的'stl'文件，以及可用于Autodesk Inventor或Fusion 360设计修改的'ipt'文件。关于该装置的更多信息，可参考发表于《Hardware X》期刊的《集成法国吸管的3D打印玻璃化装置》一文。
(1) 玻璃化棒：玻璃化质量与样本体积成反比，因为较大的热质量会导致较低的冷却速率。因此，玻璃化棒设计了多种配置，这些配置通过环路长度和高度（3D打印切片中的层数）的不同而有所区别。使用0.4毫米喷嘴打印时，每层的标称高度为0.2毫米。环路配置包括三种长度（短：15.4毫米，中：16.6毫米，长：24.4毫米）和三种高度（1层，2层，3层）。棒的总长度与法国吸管相同，以便适应标准低温储存容器。玻璃化棒采用广泛用于3D打印的聚乳酸（PLA）热塑性丝材制成，能够承受液氮温度。
(2) 制作保护套的夹具装置：设计了一种自包含的夹具装置，用于调整尺寸、压痕和切割标准0.5毫升冷冻保存吸管（IMV Technologies，法国L'Aigle）成两个保护套。该装置由一个平台、一个笔尖和一个模板组成。笔尖具有锥形尖端，可在法国吸管上产生明确的压痕，使其沿玻璃化棒上制造的法向凹槽滑动。模板确保了法国吸管在夹具中的正确定位，并促进了压痕的定位。笔尖使得在制作套件时可以定位剃刀片、笔尖和模板。使用后，笔尖和模板可插入平台中，实现自包含。