Comparative Label-Free Proteomics Study on Celiac Disease-Active Epitopes in Common Wheat, Spelt, Durum Wheat, Emmer, and Einkorn

Wheat species with
various ploidy levels may be different regarding
their immunoreactive potential in celiac disease (CD), but a comprehensive
comparison of peptide sequences with known epitopes is missing. Thus,
we used an untargeted liquid chromatography tandem mass spectrometry
method to analyze the content of peptides with CD-active epitope in
the five wheat species common wheat, spelt, durum wheat, emmer, and
einkorn. In total, 494 peptides with CD-active epitope were identified.
Considering the average of the eight cultivars of each species, spelt
contained the highest number of different peptides with CD-active
epitope (193 ± 12, mean ± SD). Einkorn showed the smallest
variability of peptides (63 ± 4) but higher amounts of certain
peptides compared to the other species. The wheat species differ in
the presence and distribution of CD-active epitopes; hence, the entirety
of peptides with CD-active epitope is crucial for the assessment of
their immunoreactive potential.