Dataset underlying publication: CarboTag: a modular approach for live and functional imaging of plant cell walls

Plant cells are contained inside a rigid network of cell walls. Cell walls are highly dynamic structures that act both as a structural material and as a hub for a wide range of signaling processes. Despite its crucial role in all aspects of the plant life cycle, live dynamical imaging of the cell wall and its functional properties has remained challenging. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag relies on a small molecular motif, a pyridine boronic acid, that targets its cargo to the cell wall, is non-toxic and ensures rapid tissue permeation. We designed a suite of cell wall imaging probes based on CarboTag in any desired color for multiplexing. Moreover, we created new functional reporters for live quantitative imaging of key cell wall features: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag opens the way to dynamical and quantitative mapping of cell wall responses at subcellular resolution.

植物细胞被包裹于刚性的细胞壁网络之中。细胞壁是一类高度动态的结构，兼具结构支撑基质与多种信号传导过程枢纽的双重功能。尽管细胞壁在植物生命周期的所有环节均发挥关键作用，但对其开展活细胞动态成像及功能特性表征仍存在诸多挑战。在此，我们介绍CarboTag——一款用于植物细胞壁活细胞功能成像的模块化工具箱。CarboTag依托一种小分子基序——吡啶硼酸（pyridine boronic acid）——实现探针向细胞壁的靶向递送，该基序无细胞毒性且可保障组织的快速渗透。我们基于CarboTag开发了一系列可按需定制荧光发色的细胞壁成像探针，可支持多通路成像。此外，我们还开发了全新的功能性报告探针，可用于核心细胞壁特性的活细胞定量成像，涵盖细胞壁网络孔隙率、细胞壁pH值以及活性氧（reactive oxygen species）的存在情况。CarboTag为在亚细胞分辨率水平下开展细胞壁响应的动态定量测绘开辟了全新路径。