Analytical sensitivity of HtLAMP-Pv, compared with other published P. vivax LAMP primers, using a DNA dilution series of clinical sample with qPCR confirmed parasitemia.

Each sample was tested in duplicate in the HtLAMP platform using each of the three P. vivax LAMP primer sets. The dilution at which both duplicates were positive was the limit of detection for each of the primer sets in the HtLAMP platform (Pos* indicates dilutions at which only one of the duplicates was positive). HtLAMP-Pv was able to detect 1.4 parasites/ μL compared with previously published P. vivax LAMP primers.