Cell death genetic signature in histotripsy treated canine Osteosarcoma cells

The objectives of the current study were to ablate three canine OS cells in-vitro with the novel focused ultrasound technique histotripsy. The cell death gene signature associated with histotripsy treatment was then evaluated 24 hours post histotripsy ablation. 3 canine osteosarcoma cell lines (Abrams, D17, and D418) were analyzed under 3 different conditions, untreated, sham treated, and histotripsy treated. This resulted in the analysis of 9 different samples which were analyzed in one single experiment. The gene GAPDH was used as the housekeeping/reference gene and gene expression patterns for the sham and histotripsy treated cells are reported relative to gene expression in untreated cells.