Celastrol-based nanomedicine hydrogels eliminate posterior capsule opacification - Supplementary table

Aim: To formulate an injectable thermosensitive micelle–hydrogel hybrid system loaded with celastrol
(celastrol-loaded micelle hydrogel: CMG) to prevent posterior capsule opacification (PCO). Materials
& methods: Celastrol-loaded micelles were embedded in a thermosensitive hydrogel matrix to enable
controlled on-demand celastrol delivery into the residual capsule. The efficacy and mechanisms of the
system for eliminating PCO were evaluated in rabbits. Results: Celastrol-loaded micelles inhibited the
migration and proliferation of lens epithelial cells induced by TGF-β1. Celastrol prevents epithelial–
mesenchymal transition in lens epithelial cells induced by TGF-β1 through the TGF-β1/Smad2/3/TEAD1
signaling pathway. In vivo efficiency evaluations showed that CMG demonstrated an excellent inhibitory
effect on PCO in rabbits and had no obvious tissue toxicity. Conclusion: Injectable CMG may represent
a promising ophthalmic platform for preventing PCO. This versatile injectable micelle–hydrogel hybrid
represents a clinically relevant platform to achieve localized therapy and controlled release of drugs in
other disease therapies.