Single-cell sequencing of FACS-sorted mouse astrocytes, investigating the effect of absence of microglia in a mouse model of beta-amyloidopathy

The APPSwe/PS1dE9 (APP/PS1) mouse beta-amyloidopathy mouse model exhibits extracellular Abeta deposition, particularly in the neocortex and hippocampus, increasing steadily from about 6 months, with reactive astrogliosis and synapse loss occurring proximal to plaques. We crossed APP/PS1 mice onto genetically modified mice which lack microglia (Csf1r deltaFIRE/deltaFIRE) to assess whether Abeta plaque deposition and downstream events are altered in brains lacking microglia.APPSwe/PS1dE9（APP/PS1）小鼠β淀粉样变性模型可出现细胞外β淀粉样蛋白（Abeta）沉积，尤以新皮层与海马体为著，该沉积自约6月龄起持续递增；斑块邻近区域可出现反应性星形胶质细胞增生与突触丢失。本研究将APP/PS1小鼠与缺失小胶质细胞的基因工程小鼠（Csf1r deltaFIRE/deltaFIRE）进行杂交，旨在评估小胶质细胞缺失的脑内，β淀粉样蛋白斑块沉积及其下游事件是否发生改变。