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Overexpression of RORA in primary mouse in vitro Th2

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https://www.ncbi.nlm.nih.gov/sra/ERP115566
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Naive CD4 T cells were taken from spleens of wild-type mice, and activated with CD3/CD28. IL4 was added to induce Th2. The cells were transduced with retrovirus to overexpress RORA vs control. Three different viral constructs were tested. M6-Rora2_mCherry appear to give the best result based on RNAseq validation of Rora expression. Rora2 = ENSMUST00000113624
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2023-10-13
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