Targeted lipidomics analysis of sphingolipid metabolism during Simkania negevensis infection

Related publication Chlamydia-like bacterium Simkania negevensis exploits host sphingolipid salvage pathway and sphingomyelin synthesis during infection Mohanty, A., Weinrich, J. D., Schumacher, F., Rühling, M., Sunuwar, S., Szegedi, H., Wigger, D., Schmelz, F., Panda, B. K., Kappe, C., Brenner, D., Schirmer, M., Arenz, C., Seibel, J., Holthuis, J. C. M., Das, S., Fraunholz, M., Kleuser, B., and Kozjak-Pavlovic, V. This record contains the source input data files for the targeted lipidomics analysis associated with the study “Chlamydia-like Bacterium Simkania negevensis Exploits Host Sphingolipids during Infection.” The files provide the lipidomics input data used for downstream analysis of sphingolipid metabolism in human cells infected with Simkania negevensis, an obligate intracellular Chlamydia-like bacterium. These data support the associated analysis workflow, including lipid abundance evaluation, heatmap generation, multivariate analysis, and statistical comparisons across experimental infection conditions and perturbation models. The corresponding analysis scripts, documentation, and workflow materials are maintained separately in the linked GitHub repository. This Zenodo archive is intended to provide a citable and persistent record of the input data underlying the targeted lipidomics analysis. This archive is intended to provide a reproducible computational record of the lipidomics analysis workflow accompanying the related publication and may also serve as a reusable template for targeted lipidomics data analysis in infection biology and host–pathogen interaction studies.