<b>The plasma membrane contributes to </b><b>temperature-induced spermatozoa motility parameters in short-term stored sperm of common carp (</b><b><i>Cyprinus carpio</i></b><b>)</b>
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Figure S1: A schematic showing experimental design of the present study.Video S1: Sperm motility and flagellum beating of demembranated aged sperm were recorded with a microscope and adaptor set at 25 fps post 16 s activation at an incubation and activation temperature of 20 ℃. Aged sperm was demembranated by a medium (45 mM NaCl, 5 mM KCl, 30 mM Tris pH 8, 1 mM EGTA, 1 mM MgCl<sub>2</sub>, 0.02% Triton X-100, 2 mg/mL BSA, 50 µL) and activated with 1.25 mM ATP (10 µL, vanadate free).Upload data: The uploaded data corresponds to all figures.
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2023-10-11
