Extracellular recordings from the locust, Schistocerca americana, olfactory pathway

This depository contains raw data from 14 experiments performed on adult locusts. The data are contained in HDF5 files (http://www.hdfgroup.org/HDF5/). They are stored as compressed integers coded on 16 bits as they came out of the A/D card. Recording details can be found in Pouzat, Mazor and Laurent (2002) Using noise signature to optimize spike-sorting and to assess neuronal classification quality. Journal of Neuroscience Methods 122: 43-57 (a pre-print version is available: http://xtof.perso.math.cnrs.fr/pdf/Pouzat+:2002.pdf). Each data file is subdivided in Groups corresponding the type of acquisition performed: one or several epochs of spontaneous activity recording; repetitive stimulation with a given odor. Each group is made of one (if say a single epoch of 60 seconds of spontaneous recording was made) or several (if say 100 stimulation with Citral were made) (sub-)groups containing the data of all the channels that were recorded during that epoch. Each of these sub-groups is made of 4 to 16 data sets: 1 dimensional arrays containing the raw data recorded from one of the 16 channels of our probe (made of 4 tetrodes) during a single acquisition epoch. All channels were sampled at 15 kHz. Each data file has attributes (metadata) README and LabBook. The first, README contains a shortened version of the present text; the second, LabBook contains a transcript of the lab book corresponding to the experiment. Most groups have a log_file_content attribute. This attribute contains a copy a text file that was automatically generated during data acquisition. Some recording details can be found there as well as the precise time and data of each recorded epoch. The data were kept for 14 years on CDs and about a third of the recordings got lost because of CD corruption! What's left still make 15 GBytes of data after compression: a substantial amount. This CD corruption explains why some groups don't have a log_file_content attribute: it was on a corrupted CD.

Of the 14 experiments, 12 contain antennal lobe (the first olfactory relay of insects) recordings, 1 contains antennal lobe and alpha lobe recordings and 1 contains only alpha lobe recordings. Here the alpha lobe location should be taken with a little bit of caution since the latter is not as easy to locate as the antennal lobe in the locust. All data files start with the locust prefix, followed by the experiment year, month and date, like locust20000214.hdf5 an experiment performed on February 14 2000. Due to file size restriction on Zenodo, two experiments are split into two data files: locust20010124b_part1.hdf5 and locust20010124b_part2.hdf5 as well as locust20010214_part1.hdf5 and locust20010214_part2.hdf5. On two dates, two different experiments were performed: locust20010124a.hdf5 and locust20010124b_part1.hdf5 / locust20010124b_part2.hdf5 as well as locust20010208a.hdf5 and locust20010208b.hdf5. When a stimulation was applied, the following code is used: Odor name / Number of stimulation / Inter-stimulation interval / duration before the odor pulse / odor pulse duration / post pulse duration [odor dilution when several dilutions were used]. All times are in seconds.

A very brief description at the group level of the files content follows (see the LabBook attribute of each individual file for details):

locust20000214.hdf5:

Citral / 70 / 30 / 3 / 0.5 / 6.5

Cherry / 120 / 30 / 3 / 0.5 / 6.5

Octaldehyde / 60 / 30 / 3 / 0.5 / 6.5

locust20000421.hdf5:

Spontaneous: 60 seconds of spontaneous activity

1-Hexanol / 30 / 10 / 3 / 1 / 5.5

Hexanal / 25 / 10 / 3 / 1 / 5.5

Cis-3-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5

Trans-2-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5

1-Hexen-3-ol / 25 / 10 / 3 / 1 / 5.5

3-Pentanone / 25 / 10 / 3 / 1 / 5.5

1-Heptanol / 25 / 10 / 3 / 1 / 5.5

1-Octanol / 25 / 10 / 3 / 1 / 5.5

2-Heptanone / 25 / 10 / 3 / 1 / 5.5

3-Heptanone / 25 / 10 / 3 / 1 / 5.5

Citral / 25 / 10 / 3 / 1 / 5.5

Apple / 25 / 10 / 3 / 1 / 5.5

Mint / 25 / 10 / 3 / 1 / 5.5

Strawberry / 25 / 10 / 3 / 1 / 5.5

Octaldehyde / 25 / 10 / 3 / 1 / 5.5

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-5]

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-4]

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-3]

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-2]

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [10^-1]

1-Octanol / 25 / 10 / 3 / 1 / 5.5 [1]

locust20000423.hdf5:

Spontaneous first: 60 seconds of spontaneous activity

1-Hexanol / 25 / 10 / 3 / 1 / 5.5

Hexanal / 25 / 10 / 3 / 1 / 5.5

Cis-3-hexen-1-ol / 25 / 10 / 3 / 1 / 5.5

1-Hexen-3-ol / 25 / 10 / 3 / 1 / 5.5

1-Heptanol / 25 / 10 / 3 / 1 / 5.5

2-Heptanone / 25 / 10 / 3 / 1 / 5.5

3-Heptanone / 25 / 10 / 3 / 1 / 5.5

Citral / 25 / 10 / 3 / 1 / 5.5

Apple / 25 / 10 / 3 / 1 / 5.5

Amyl Acetate / 25 / 10 / 3 / 1 / 5.5

1-Hexanol / 25 / 10 / 3 / 1 / 5.5

Spontaneous second: 60 seconds of spontaneous activity

locust20000613.hdf5:

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]

Cis-3-hexen-1-ol / 10 / 30 / 3 / 1 / 16 [1/100]

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/10]

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]

Cherry / 21 / 30 / 3 / 1 / 16

locust20000616.hdf5:

Spontaneous first: 60 seconds of spontaneous activity

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1]

Spontaneous second: 60 seconds of spontaneous activity

Spontaneous third: 60 seconds of spontaneous activity

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/100]

Cis-3-hexen-1-ol / 50 / 30 / 3 / 1 / 16 [1/10]

locust20000901.hdf5:

Vanilla / 5 / 30 / 3 / 1 / 16

Spontaneous: 60 seconds of spontaneous activity

Cherry / 30 / 30 / 3 / 1 / 16

Spontaneous: 60 seconds of spontaneous activity

Benzaldehyde / 30 / 30 / 3 / 1 / 16

Spontaneous: 60 seconds of spontaneous activity

Mint / 20 / 30 / 3 / 1 / 16

Hexanal / 15 / 30 / 3 / 1 / 16

Spontaneous: 60 seconds of spontaneous activity

Cis-3-hexen-1-ol / 30 / 30 / 3 / 1 / 16

Spontaneous: 60 seconds of spontaneous activity

Trans-2-hexen-1-ol / 30 / 30 / 3 / 1 / 16

locust20010124a.hdf5:

Spontaneous: 2x29 seconds of spontaneous activity

Spontaneous: 60x29 seconds of spontaneous activity

Spontaneous: 80x29 seconds of spontaneous activity

locust20010124b_part1.hdf5 and locust20010124b_part2.hdf5:

Spontaneous: 60x29 seconds of spontaneous activity

Spontaneous: 191x29 seconds of spontaneous activity

Spontaneous: 59x29 seconds of spontaneous activity

locust20010131.hdf5:

Spontaneous: 90x29 seconds of spontaneous activity

Spontaneous: 70x29 seconds of spontaneous activity

Spontaneous: 5x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

Spontaneous: 3x59 seconds of spontaneous activity

WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)

WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)

WithoutAntenna: 3x59 seconds of spontaneous activity (after antennal nerve cut)

locust20010201:

Continuous: 90x29 seconds of spontaneous activity

Continuous: 20x29 seconds of spontaneous activity

Citral / 50 / 30 / 3 / 1 / 25

Citral / 50 / 30 / 10 / 1 / 18

Citral / 50 / 30 / 10 / 1 / 18

Continuous: 50x29 seconds of spontaneous activity

Continuous: 45x29 seconds of spontaneous activity

locust20010208a.hdf5:

Spontaneous: 50x29 seconds of spontaneous activity

Spontaneous: 80x29 seconds of spontaneous activity

locust20010208b.hdf5:

Spontaneous: 50x29 seconds of spontaneous activity

Spontaneous: 50x29 seconds of spontaneous activity

Citral / 50 / 30 / 10 / 1 / 18

Citral / 120 / 30 / 10 / 1 / 18

Citral / 50 / 30 / 10 / 1 / 18

Citral / 25 / 30 / 10 / 1 / 18

locust20010214_part1.hdf5 and locust20010214_part2.hdf5:

Spontaneous: 30x29 seconds of spontaneous activity

Spontaneous: 30x29 seconds of spontaneous activity

Cis-3-hexen-1-ol / 25 / 30 / 10 / 1 / 18

Citral / 25 / 30 / 10 / 1 / 18

Vanilla / 25 / 30 / 10 / 1 / 18

Octanol / 25 / 30 / 10 / 1 / 18

Mint / 25 / 30 / 10 / 1 / 18

Cis-3-hexen-1-ol / 25 / 30 / 10 / 1 / 18

Spontaneous: 30x29 seconds of spontaneous activity

Spontaneous: 30x29 seconds of spontaneous activity

Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18

Cis-3-hexen-1-ol / 11 / 30 / 10 / 1 / 18

Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18

Cis-3-hexen-1-ol / 30 / 30 / 10 / 1 / 18

locust20010217.hdf5:

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 2x29 seconds of spontaneous activity

Spontaneous: 30x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity

Spontaneous: 10x29 seconds of spontaneous activity