Impact of the bone microenvironment on phenotypic plasticity of ER+ breast cancer cells [Evolving Barcode]

Evolving barcode was used to evaluate clonal expansion in bone metastases induced by intra-iliac artery injection. The assocoation of these results with ER expression revealed a non-genomic effect of the bone microenvironment on ER heterogeneity. Overall design: ER-positive breast cancer cells (MCF7) with hgRNA-A21 and inducible Cas9 expression were inejcted to hind limb of nude mice. Cas9 expression was induced weekly and laser capture microdissection (LCM) was employed to collect independent bone lesions for targeted sequencing at week 3. All samples were processed using the TraceQC package