Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

Next generation sequencing (NGS) and bioinformatics were performed to analysis and annotate the expression profiles and functions of exosome-derived circRNAs in pancreatic cells during radiation. A total of 12572 circRNAs were identified, and 3580 circRNAs were annotated in literatures and circBase. There were 196 filtered differentially expressed circRNAs (182 for up-regulated and 14 for down-regulated, fold change > 2, p-value < 0.05). GO and KEGG analyses indicated that regulation of metabolic process and lysine degradation were the main biological processes and pathway enrichment. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing. The hsa_circ_0002130-hsa-miR-4482-3p-NBN interaction network suggested potential miRNA sponges and target mRNAs. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell upon irradiation. Examination of total RNA profiles of pancreatic cells-derived exosomes by NGS