Video recording of freely diffusing 100nm polystyrene nanoparticles using dark field microscopy

Dark field recordings of 100nm polystyrene (Kisker Biotech PPs-01) particles which were diluted in essentially particle-free double distilled H2O. The temperature was 24 °C. The concentration was amounted to approximately 3x10^9 ml. The video file was captured with a PCO Edge CCD camera at 30 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser. The video pixel size is 63nm. Please note that the video is splitted into mutiple archives. Please download all archives to get the full video. <strong>This video is part of a puplication:</strong> <strong>Wagner, T., Lipinski, H.-G. &amp; Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong>