Unraveling the inactivation mechanisms of human adenovirus 2 in sunlight disinfection: Synergism between direct and indirect pathways

Data files for the figures in manuscript titled: "Unraveling the inactivation mechanisms of human adenovirus 2 in sunlight disinfection: Synergism between direct and indirect pathways" submitted to ACS Environmental Science & Technology

Experimental data

This folder contains the experimental data to the figures shown in the main manuscript and Supporting Information.

Figure 1: measured and predicted inactivation rate constants of HAdV2 in SRNOM-spiked buffer exposed to simulated sunlight.

Figure 2: measured inactivation kinetics of HAdV2 under simulated sunlight following different levels of singlet oxygen pre-exposures.

Figure 3: measured inactivation kinetics of HAdV2 in A549 and XP17BE under simulated sunlight.

Figure 4: monitoring of HAdV2 genome copies at 2 and 48 hours of incubation. 

Figure S1: simulated solar spectrum generated using AM 1.5 and atmospheric edge filter with and without glass Petridish cover under identical intensity settings.

Figure S2: solar inactivation of HAdV2 with a reactor covered by a glass Petri dish, with and without Rose Bengal.

Figure S3: photo bleaching of Rose Bengal under full-spectrum simulated sunlight with a glass Petri dish cover and measured steady-state concentration of singlet oxygen.

Figure S4: standard curves of HAdV2 hexon gene 1.1 kbp LR-PCR products targeting 177 bp and human beta actin genes targeting 103 bp amplicons.

Figure S5: HAdV2 hexon gene monitoring at 0 hour and 48 hours of incubation.

Figure S6: dark control of HAdV2 irradiated with 15 mgC/L of SRNOM up to 48 hours covered with aluminum foil.

Figure S7: solar inactivation kinetics of singlet oxygen pre-exposed HAdV2 samples.

Figure S8: HAdV2 inactivation and its genome degradation under direct system and singlet oxygen dominant system.

Abbrevations used:

HAdV2 = Human adenovirus 2

LR-PCR = Long range polymerase cycle reaction

ml = mililiter

MPN = Most probable number

SRNOM = Suwanee river natural organic matter