Primer sequences used for cloning of NS4 ORF into pGEX-4T-2 or pCI-neo.

Underlined sequences are specific to the NS4 ORF. Sequences in italics in the reverse primers indicate two successive stop codons. Sequences in bold characters are restriction enzyme sites (in NS4-BTVfor and NS4-GIVfor, the site is EcoRI; in NS4-BTVrev the site is NotI, in NS4-GIVrev-pGEX the site is XhoI and in NS4-GIVrev-pCI the site is XbaI). Sequences in lower case characters are non-specific nucleotides added for an efficient restriction enzyme digestion.